One of the world's rarest turtles, a Yangtze giant softshell, has died in China, leaving just three remaining, BBC reports.
Also known as Rafetus swinhoei, the female turtle died in the Suzhou zoo in southern China.
Experts had tried to artificially inseminate the creature, which was over 90 years old, for a fifth time shortly before she died.
The species is critically endangered due to hunting, overfishing and the destruction of its habitat.
One male is left in the Chinese zoo while two other turtles live in the wild in Vietnam. The illusive nature of the turtle means it has been difficult to identify the latter's gender.